Journal: Antimicrobial Agents and Chemotherapy
Article Title: Treatment with Bumped Kinase Inhibitor 1294 Is Safe and Leads to Significant Protection against Abortion and Vertical Transmission in Sheep Experimentally Infected with Toxoplasma gondii during Pregnancy
doi: 10.1128/AAC.02527-18
Figure Lengend Snippet: IgG responses in sera by ELISA based on T. gondii SAG1 protein (A) or soluble antigens (B). Values from infected groups (G1 and G2) and the group receiving vehicle alone (G4) are shown. Each point represents the means ± SD at the different sampling times for each group.
Article Snippet: Fifteen micrograms of T. gondii BAG1 recombinant protein expressed in Escherichia coli (GenScript, Nanjing, China) was electrophoresed in 15% polyacrylamide-DATD minigels and transferred to nitrocellulose membranes (Mini Trans-Blot cell; Bio-Rad Laboratories, CA, USA).
Techniques: Enzyme-linked Immunosorbent Assay, Infection, Sampling